It is a type of biochemical test which is used for the identification of Staphylococcus, Enterococcus, and other Gram-positive bacilli.This technique was discovered to be quicker than the gelatin stab technique. In this technique, gelatin hydrolysis was seen when charcoal particles were released and resolved to the bottom of the culture tube. Green and Larks (1955) also published a quick technique for the identification of gelatin-liquefying bacteria applying formalin-denatured gelatin-charcoal. This observation revealed that hydrolysis of gelatin was usually more reliable and rapid in the simplified plate technique(3 days) than in the stab method (up to 14 days) and Frazier’s plate method (up to 4 days).Later Clarke (1953) reported a simplified plate technique applying 10% leaf gelatin and HgCl 2–HCl solution for the identification of gelatin-liquefying bacteria and compared it with the gelatin stab method and Frazier’s plate method.The method for gelatin hydrolysis was then listed as one of the biochemical tests for bacteriology. The method was tested against isolated intestinal Gram-negative bacilli by Barer and against aerobic spore-forming bacilli by Smith, Gordon, and Clarke in 1946 as mentioned by Clarke.Then the plates are treated with acidic mercuric chloride or tannic acid to detect the digestion of gelatin. In this technique, the microorganisms are grown on a low-peptone agar medium supplemented with gelatin.The technique by which we can detect the ability of microorganisms to liquefy gelatin was first described by Frazier, in 1926.Organisms that produce proteolytic enzymes (gelatinases) hydrolyze gelatin to make polypeptides or individual amino acids. For a long time, gelatin has been used in food solidification. Gelatin is a protein that comes from animal collagen, a component of the vertebrate connective tissues.
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